Detection of Escherichia coli and Pseudomonas aeruginosa using REP-PCR monitoring goat´s milk quality in milking room

Chapaval, Lea; Olivindo, Cellyneude de Souza; Souza, Francisca Geovânia Canafístula de; Alves, Francisco Selmo Fernandes; Frota, Isana Mara Aragão

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dc.creator	Chapaval, Lea
dc.creator	Olivindo, Cellyneude de Souza
dc.creator	Souza, Francisca Geovânia Canafístula de
dc.creator	Alves, Francisco Selmo Fernandes
dc.creator	Frota, Isana Mara Aragão
dc.date	2010-04-01
dc.date.accessioned	2022-03-21T20:06:53Z
dc.date.available	2022-03-21T20:06:53Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/9
dc.identifier	10.14295/cs.v1i1.9
dc.identifier.uri	http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77472
dc.description	The present study was carried out with the objective of applying the REP-PCR sequences on the monitoring of goat milk quality, through the detection of Escherichia coli and Pseudomonas aeruginosa, in samples of milking handlers, goat teats, milk, milk machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points. Several fingerprints were verified of all isolates collected of the different studied sources (milking handlers, goat teats, milk, milk machine and water). It was observed very similar behaviors of the bands indicating that the isolates can be related as epidemic clones. Hands of the milking handlers were characterized as a critical point of control, because they stand out as initial point of Escherichia coli and Pseudomonas aeruginosa contamination. The technique demonstrated to be efficient for the similarity analysis among individuals of the Escherichia coli and Pseudomonas aeruginosa species, being, therefore, an useful tool for investigation of fails on management and consequently, in the search of more efficient control to avoid or to minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals, and can be transmitted through products such as milk and your products.	en-US
dc.description	O presente estudo foi realizado com o objetivo de aplicar a técnica de REP-PCR no monitoramento da qualidade do leite de cabra, através da detecção de Escherichia coli e Pseudomonas aeruginosa, em amostras de mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água, para o futuro estabelecimento e implantação do sistema de Análise de Perigos e Pontos Críticos de Controle. Verificaram-se vários fingerprints de todos os isolados coletados das diferentes fontes estudadas (mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água). Observaram-se comportamentos muito similares das bandas indicando que os isolados podem ser relatados como clones epidemiológicos. As mãos do ordenhador caracterizaram-se como ponto crítico de controle, pois se destaca como iniciador de contaminação nas amostras de Escherichia coli e Pseudomonas aeruginosa. A técnica demonstrou ser eficiente para a análise da similaridade entre indivíduos das espécies estudadas, sendo, portanto, uma ferramenta útil para investigação de falhas no manejo e consequentemente, na busca de um controle mais eficiente para evitar ou minimizar a disseminação de microrganismos patogênicos causadores de sérias enfermidades em humanos e animais, que muitas vezes podem ser transmitidas através de produtos como o leite e seus derivados.Detection of Escherichia coli and Pseudomonas aeruginosa using REP-PCR monitoring goat´s milk quality in milking roomAbstractThe present study was carried out with the objective of applying the REP-PCR sequences on the monitoring of goat milk quality, through the detection of Escherichia coli and Pseudomonas aeruginosa, in samples of milking handlers, goat teats, milk, milk machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points. Several fingerprints were verified of all isolates collected of the different studied sources (milking handlers, goat teats, milk, milk machine and water). It was observed very similar behaviors of the bands indicating that the isolates can be related as epidemic clones. Hands of the milking handlers were characterized as a critical point of control, because they stand out as initial point of Escherichia coli and Pseudomonas aeruginosa contamination. The technique demonstrated to be efficient for the similarity analysis among individuals of the Escherichia coli and Pseudomonas aeruginosa species, being, therefore, an useful tool for investigation of fails on management and consequently, in the search of more efficient control to avoid or to minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals, and can be transmitted through products such as milk and your products.	pt-BR
dc.format	application/pdf
dc.language	por
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/9/21
dc.source	Comunicata Scientiae; Vol. 1 No. 1 (2010); 49	en-US
dc.source	Comunicata Scientiae; v. 1 n. 1 (2010); 49	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.source	10.14295/cs.v1i1
dc.subject	environmental microrganisms	en-US
dc.subject	molecular epidemiology	en-US
dc.subject	milk quality.	en-US
dc.subject	microrganismos ambientais	pt-BR
dc.subject	epidemiologia molecular	pt-BR
dc.subject	qualidade do leite	pt-BR
dc.title	Detection of Escherichia coli and Pseudomonas aeruginosa using REP-PCR monitoring goat´s milk quality in milking room	en-US
dc.title	Detecção de Escherichia coli e Pseudomonas aeruginosa pela técnica de REP-PCR no monitoramento da qualidade do leite de cabra em sala de ordenha	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion