The present study was carried out with the objective of applying the REP-PCR sequences on the monitoring of goat milk quality, through the detection of Escherichia coli and Pseudomonas aeruginosa, in samples of milking handlers, goat teats, milk, milk machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points. Several fingerprints were verified of all isolates collected of the different studied sources (milking handlers, goat teats, milk, milk machine and water). It was observed very similar behaviors of the bands indicating that the isolates can be related as epidemic clones. Hands of the milking handlers were characterized as a critical point of control, because they stand out as initial point of Escherichia coli and Pseudomonas aeruginosa contamination. The technique demonstrated to be efficient for the similarity analysis among individuals of the Escherichia coli and Pseudomonas aeruginosa species, being, therefore, an useful tool for investigation of fails on management and consequently, in the search of more efficient control to avoid or to minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals, and can be transmitted through products such as milk and your products.  O presente estudo foi realizado com o objetivo de aplicar a técnica de REP-PCR no monitoramento da qualidade do leite de cabra, através da detecção de Escherichia coli e Pseudomonas aeruginosa, em amostras de mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água, para o futuro estabelecimento e implantação do sistema de Análise de Perigos e Pontos Críticos de Controle. Verificaram-se vários fingerprints de todos os isolados coletados das diferentes fontes estudadas (mãos de ordenhador, tetos das cabras, leite, ordenhadeira e água). Observaram-se comportamentos muito similares das bandas indicando que os isolados podem ser relatados como clones epidemiológicos. As mãos do ordenhador caracterizaram-se como ponto crítico de controle, pois se destaca como iniciador de contaminação nas amostras de Escherichia coli e Pseudomonas aeruginosa. A técnica demonstrou ser eficiente para a análise da similaridade entre indivíduos das espécies estudadas, sendo, portanto, uma ferramenta útil para investigação de falhas no manejo e consequentemente, na busca de um controle mais eficiente para evitar ou minimizar a disseminação de microrganismos patogênicos causadores de sérias enfermidades em humanos e animais, que muitas vezes podem ser transmitidas através de produtos como o leite e seus derivados.Detection of Escherichia coli and Pseudomonas aeruginosa using REP-PCR monitoring goat´s milk quality in milking roomAbstractThe present study was carried out with the objective of applying the REP-PCR sequences on the monitoring of goat milk quality, through the detection of Escherichia coli and Pseudomonas aeruginosa, in samples of milking handlers, goat teats, milk, milk machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points. Several fingerprints were verified of all isolates collected of the different studied sources (milking handlers, goat teats, milk, milk machine and water). It was observed very similar behaviors of the bands indicating that the isolates can be related as epidemic clones. Hands of the milking handlers were characterized as a critical point of control, because they stand out as initial point of Escherichia coli and Pseudomonas aeruginosa contamination. The technique demonstrated to be efficient for the similarity analysis among individuals of the Escherichia coli and Pseudomonas aeruginosa species, being, therefore, an useful tool for investigation of fails on management and consequently, in the search of more efficient control to avoid or to minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals, and can be transmitted through products such as milk and your products.