Molecular Characterization of a Novel Begomovirus Infecting Malva (Gaya guerkeana K. Schum.)

Ferro, Mayra Machado de Medeiros; Tenório, Arthur Alencar Raposo; Silva, Sarah Jacqueline Cavalcanti da; Silva, Janaíne Rossane Araújo; Ramos-Sobrinho, Roberto; Lima, Gaus Silvestre de Andrade; Assunção, Iraildes Pereira

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dc.creator	Ferro, Mayra Machado de Medeiros
dc.creator	Tenório, Arthur Alencar Raposo
dc.creator	Silva, Sarah Jacqueline Cavalcanti da
dc.creator	Silva, Janaíne Rossane Araújo
dc.creator	Ramos-Sobrinho, Roberto
dc.creator	Lima, Gaus Silvestre de Andrade
dc.creator	Assunção, Iraildes Pereira
dc.date	2018-11-04
dc.date.accessioned	2022-03-21T20:08:08Z
dc.date.available	2022-03-21T20:08:08Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/2083
dc.identifier	10.14295/cs.v9i3.2083
dc.identifier.uri	http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77883
dc.description	The genus Begomovirus (family Geminirvidae) comprises circular single-stranded DNA viruses that infect both cultivated and non-cultivated hosts, being transmitted by the insect vector Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). This study aimed to conduct the molecular characterization of a novel begomovirus infecting the non-cultivated plant Gaya guerkeana K. Schum. (Malvaceae). A leaf sample of G. guerkeana was collected in the State of Pernambuco, Brazil, in 2010. Viral genomic components were amplified via RCA, cloned, and commercially sequenced. The full-length DNA-A sequence was used for phylogenetic analysis and pairwise comparisons with other previously reported begomoviruses. The begomovirus DNA-A from G. guerkeana was most closely related to the Sida mottle Alagoas virus (SiMoAV, JX871386), having 84.8% nucleotide identity. Using the ≥ 91% identity criteria for DNA-A established by the Geminivirus Study Group of the International Committee on Taxonomy of Viruses, this isolate was considered a putative new begomovirus species, and the name Gaya yellow mosaic virus (GaYMV) is suggested. The GaYMV isolate grouped with SiMoAV and other begomoviruses infecting Sida spp. from the State of Alagoas, Brazil. This is the first report of a begomovirus infecting G. guerkeana, indicating that this non-cultivated plant can act as a reservoir/source of begomoviruses.	en-US
dc.description	The genus Begomovirus (family Geminirvidae) comprises circular single-stranded DNA viruses that infect both cultivated and non-cultivated hosts, being transmitted by the insect vector Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). This study aimed to conduct the molecular characterization of a novel begomovirus infecting the non-cultivated plant Gaya guerkeana K. Schum. (Malvaceae). A leaf sample of G. guerkeana was collected in the State of Pernambuco, Brazil, in 2010. Viral genomic components were amplified via RCA, cloned, and commercially sequenced. The full-length DNA-A sequence was used for phylogenetic analysis and pairwise comparisons with other previously reported begomoviruses. The begomovirus DNA-A from G. guerkeana was most closely related to the Sida mottle Alagoas virus (SiMoAV, JX871386), having 84.8% nucleotide identity. Using the ≥ 91% identity criteria for DNA-A established by the Geminivirus Study Group of the International Committee on Taxonomy of Viruses, this isolate was considered a putative new begomovirus species, and the name Gaya yellow mosaic virus (GaYMV) is suggested. The GaYMV isolate grouped with SiMoAV and other begomoviruses infecting Sida spp. from the State of Alagoas, Brazil. This is the first report of a begomovirus infecting G. guerkeana, indicating that this non-cultivated plant can act as a reservoir/source of begomoviruses.	pt-BR
dc.format	application/pdf
dc.format	application/msword
dc.format	application/pdf
dc.language	eng
dc.language	por
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2083/587
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2083/745
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2083/746
dc.rights	Copyright (c) 2018 Mayra Machado de Medeiros Ferro, Arthur Alencar Raposo Tenório, Sarah Jacqueline Cavalcanti da Silva, Janaíne Rossane Araújo Silva, Roberto Ramos-Sobrinho, Iraildes Pereira Assunção	en-US
dc.source	Comunicata Scientiae; Vol. 9 No. 3 (2018); 527-530	en-US
dc.source	Comunicata Scientiae; v. 9 n. 3 (2018); 527-530	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.subject	alternative host	pt-BR
dc.subject	Gaya guerkeana	pt-BR
dc.subject	geminivirus	pt-BR
dc.subject	phylogeny	pt-BR
dc.title	Molecular Characterization of a Novel Begomovirus Infecting Malva (Gaya guerkeana K. Schum.)	en-US
dc.title	Molecular Characterization of a Novel Begomovirus Infecting Malva (Gaya guerkeana K. Schum.)	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.type	Molecular characterization; Etiology	pt-BR