Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality

Pegoraro, Camila; Storch, Tatiane Timm; Crizel, Giseli Rodrigues; Rombaldi, Cesar Valmor; Girardi, César Luis

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dc.creator	Pegoraro, Camila
dc.creator	Storch, Tatiane Timm
dc.creator	Crizel, Giseli Rodrigues
dc.creator	Rombaldi, Cesar Valmor
dc.creator	Girardi, César Luis
dc.date	2017-02-23
dc.date.accessioned	2022-03-21T20:07:52Z
dc.date.available	2022-03-21T20:07:52Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/1545
dc.identifier	10.14295/cs.v7i4.1545
dc.identifier.uri	http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77821
dc.description	Apple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period.	en-US
dc.description	Apple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period.	pt-BR
dc.format	application/pdf
dc.language	eng
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/1545/434
dc.rights	Copyright (c) 2017 Camila Pegoraro, Tatiane Timm Storch, Giseli Rodrigues Crizel, Cesar Valmor Rombaldi, César Luis Girardi	en-US
dc.source	Comunicata Scientiae; Vol. 7 No. 4 (2016); 494-503	en-US
dc.source	Comunicata Scientiae; v. 7 n. 4 (2016); 494-503	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.subject	Cold storage	en-US
dc.subject	gene expression	en-US
dc.subject	Malus x domestica	en-US
dc.subject	molecular analysis	en-US
dc.title	Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality	en-US
dc.title	Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.type	Artigo Científico	en-US