Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins

Freitas, Cleverson Diniz; Ramos, Marcio Viana; Souza, Diego Pereira; Marinho-Filho, Jose Delano Barreto; Teixeira, Fabiano Moura; Oliveira, Jefferson Soares

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dc.creator	Freitas, Cleverson Diniz
dc.creator	Ramos, Marcio Viana
dc.creator	Souza, Diego Pereira
dc.creator	Marinho-Filho, Jose Delano Barreto
dc.creator	Teixeira, Fabiano Moura
dc.creator	Oliveira, Jefferson Soares
dc.date	2011-04-19
dc.date.accessioned	2022-03-21T20:06:53Z
dc.date.available	2022-03-21T20:06:53Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/15
dc.identifier	10.14295/cs.v2i1.15
dc.identifier.uri	http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77477
dc.description	Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed.	en-US
dc.description	Lectinas purificadas de sementes de Canavalia brasiliensis (ConBr) e Cratylia floribunda (CFL) foram testadas quanto a atividade inseticida sobre Callosobruchus maculatus (Coleoptera: Bruchidae) e Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr e CFL causaram efeitos negativos sobre o desenvolvimento das larvas de C. maculatus nas concentrações de 1% e 2% (p/p). O tempo de desenvolvimento dos insetos crescidos nas sementes artificiais contendo ConBr ou CFL não foi significativamente alterado, entretanto a porcentagem de emergência foi diminuída. Ambas lectinas foram resistentes a ação hidrolítica de proteases intestinais de larvas de C. maculatus. Dietas artificiais contendo as lectinas não causaram efeitos deletérios sobre a massa e a sobrevivência de ninfas de D. peruvianus. Proteases intestinais extraídas de D. peruvianus foram capazes de hidrolisar ConBr e CFL após 30 minutos de incubação. A habilidade dos insetos para digerir as lectinas parece estar diretamente relacionada ao efeito inseticida observado. Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/Mannose Plant Lectins AbstractPurified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed.	pt-BR
dc.format	application/pdf
dc.language	por
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/15/57
dc.source	Comunicata Scientiae; Vol. 2 No. 1 (2011); 34-41	en-US
dc.source	Comunicata Scientiae; v. 2 n. 1 (2011); 34-41	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.subject	Carbohydrate-binding proteins	en-US
dc.subject	Callosobruchus maculatus	en-US
dc.subject	Dysdercus peruvianus	en-US
dc.subject	lectins.	en-US
dc.subject	Proteínas ligantes a carboidratos	pt-BR
dc.subject	Callosobruchus maculatus	pt-BR
dc.subject	Dysdercus peruvianus	pt-BR
dc.subject	Pragas agrícolas.	pt-BR
dc.title	Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins	en-US
dc.title	Correlações entre atividade inseticida e resistência a proteólise de duas lectinas vegetais glicose/manose	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion