Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization

Ribeiro, Camila Vasconcelos; Neves, Tábatta Arrivabene; Fagundes, Glaucia Brandão; do Nascimento, Dayana Maria; da Silva, Cleidson Manoel Gomes; Arrivabene, Mônica; Dias, Francisca Elda Ferreira; Cavalcante, Tânia Vasconcelos

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dc.creator	Ribeiro, Camila Vasconcelos
dc.creator	Neves, Tábatta Arrivabene
dc.creator	Fagundes, Glaucia Brandão
dc.creator	do Nascimento, Dayana Maria
dc.creator	da Silva, Cleidson Manoel Gomes
dc.creator	Arrivabene, Mônica
dc.creator	Dias, Francisca Elda Ferreira
dc.creator	Cavalcante, Tânia Vasconcelos
dc.date	2019-04-17
dc.date.accessioned	2023-03-30T19:35:11Z
dc.date.available	2023-03-30T19:35:11Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/2756
dc.identifier	10.14295/cs.v10i1.2756
dc.identifier.uri	https://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/246806
dc.description	Vaginal cytology analysis has been used to evaluate the different stages of estrous cycle of several species; it presents a direct correlation with the animal’s hormonal state and provides essential information about the female reproductive tract conditions. Two staining methods were tested to evaluate the vaginal epithelial cell morphology of nulliparous and multiparous ewes during the estrus period. An intravaginal device impregnated with medroxyprogesterone acetate was kept into 10 nulliparous and 10 multiparous ewes for 14 days for estrus synchronization. Then, the progesterone device was withdrawn, and 300 IU of eCG was administered intramuscularly. Vaginal smears were prepared for posterior staining with Panotico or Giemsa stains when estrus was detected. The cells were classified into nucleated superficial, anucleate superficial, intermediate, parabasal, and basal. The Panotico and Giemsa staining of the different cell types studied were satisfactory. A predominance of intermediate epithelial cells (p<0.05) was found after staining. No difference in percentages of the different types of vaginal epithelial cells between nulliparous and multiparous ewes were found. Therefore, both staining methods were efficient, and a predominance of intermediate cells is found in nulliparous and multiparous ewes during the estrus period.	en-US
dc.description	Vaginal cytology analysis has been used to evaluate the different stages of estrous cycle of several species; it presents a direct correlation with the animal’s hormonal state and provides essential information about the female reproductive tract conditions. Two staining methods were tested to evaluate the vaginal epithelial cell morphology of nulliparous and multiparous ewes during the estrus period. An intravaginal device impregnated with medroxyprogesterone acetate was kept into 10 nulliparous and 10 multiparous ewes for 14 days for estrus synchronization. Then, the progesterone device was withdrawn, and 300 IU of eCG was administered intramuscularly. Vaginal smears were prepared for posterior staining with Panotico or Giemsa stains when estrus was detected. The cells were classified into nucleated superficial, anucleate superficial, intermediate, parabasal, and basal. The Panotico and Giemsa staining of the different cell types studied were satisfactory. A predominance of intermediate epithelial cells (p<0.05) was found after staining. No difference in percentages of the different types of vaginal epithelial cells between nulliparous and multiparous ewes were found. Therefore, both staining methods were efficient, and a predominance of intermediate cells is found in nulliparous and multiparous ewes during the estrus period.	pt-BR
dc.format	application/pdf
dc.language	eng
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2756/784
dc.rights	Copyright (c) 2019 Camila Vasconcelos Ribeiro, Tábatta Arrivabene Neves, Glaucia Brandão Fagundes, Dayana Maria do Nascimento, Cleidson Manoel Gomes da Silva, Mônica Arrivabene, Francisca Elda Ferreira Dias, Tânia Vasconcelos Cavalcante	en-US
dc.source	Comunicata Scientiae; Vol. 10 No. 1 (2019); 5-9	en-US
dc.source	Comunicata Scientiae; v. 10 n. 1 (2019); 5-9	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.title	Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization	en-US
dc.title	Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion