Extracts of Tagetes minuta L. front of bacteria regarding  bovine mastitis

Berguenmaier de Olanda, Gabriela; Peripolli Bevilaqua, Gilberto Antônio; Damé Schuch, Luiz Filipe; Souza Prestes, Luciana; Batista Job, Ricardo

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dc.creator	Berguenmaier de Olanda, Gabriela
dc.creator	Peripolli Bevilaqua, Gilberto Antônio
dc.creator	Damé Schuch, Luiz Filipe
dc.creator	Souza Prestes, Luciana
dc.creator	Batista Job, Ricardo
dc.date	2019-04-17
dc.date.accessioned	2023-03-30T19:35:06Z
dc.date.available	2023-03-30T19:35:06Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/2623
dc.identifier	10.14295/cs.v10i1.2623
dc.identifier.uri	https://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/246782
dc.description	Bovine mastitis is a problem in the context of animal health. However medicinal plants are a management strategy in agroecological production systems. In this context, the plant Tagetes minuta L. plays a leading role, due to its recognition in the popular pharmacopeia. Thus, the objective of this work was to assess the effect of hydroalcoholic extracts of T. minuta from different collection sites compared to ten bacteria related to bovine mastitis. The antimicrobial evaluation was done through the microdilution technique in syrup, in 96 well microplates in triplicate, for the determination of Minimal Bacterial Concentration (MBC) (%). In the microplates, the extracts of T. minuta and the inoculums of the bacteria were placed in addition to the growth control of the bacteria and the control of the extracts, which were incubated for 72 hours at 37°C in an oven. After this time, aliquots of 5μL of each orifice were transferred to plates containing 5% equine defibrinated blood agar and maintained in incubation for 24 hours at the same previous temperature. With this, the growth readings were performed, with the subsequent interpretation of the results by the geometric mean of the MBC (%). The results indicate that there is a difference in sensitivity to microorganisms by the extracts as a function of the collection sites and both present antimicrobial action.	en-US
dc.description	Bovine mastitis is a problem in the context of animal health. However medicinal plants are a management strategy in agroecological production systems. In this context, the plant Tagetes minuta L. plays a leading role, due to its recognition in the popular pharmacopeia. Thus, the objective of this work was to assess the effect of hydroalcoholic extracts of T. minuta from different collection sites compared to ten bacteria related to bovine mastitis. The antimicrobial evaluation was done through the microdilution technique in syrup, in 96 well microplates in triplicate, for the determination of Minimal Bacterial Concentration (MBC) (%). In the microplates, the extracts of T. minuta and the inoculums of the bacteria were placed in addition to the growth control of the bacteria and the control of the extracts, which were incubated for 72 hours at 37°C in an oven. After this time, aliquots of 5μL of each orifice were transferred to plates containing 5% equine defibrinated blood agar and maintained in incubation for 24 hours at the same previous temperature. With this, the growth readings were performed, with the subsequent interpretation of the results by the geometric mean of the MBC (%). The results indicate that there is a difference in sensitivity to microorganisms by the extracts as a function of the collection sites and both present antimicrobial action.	pt-BR
dc.format	application/pdf
dc.language	eng
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2623/783
dc.rights	Copyright (c) 2019 Gabriela Berguenmaier de Olanda, Gilberto Antônio Peripolli Bevilaqua, Luiz Filipe Damé Schuch, Luciana Souza Prestes, Ricardo Batista Job	en-US
dc.source	Comunicata Scientiae; Vol. 10 No. 1 (2019); 1-4	en-US
dc.source	Comunicata Scientiae; v. 10 n. 1 (2019); 1-4	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.title	Extracts of Tagetes minuta L. front of bacteria regarding bovine mastitis	en-US
dc.title	Extracts of Tagetes minuta L. front of bacteria regarding bovine mastitis	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion