In vitro cultivation of Tamarindus indica L.:  explants obtention and contamination in culture medium

Ferreira, Antonio Flávio Arruda; Monteiro, Laís Naiara Honorato; Rodrigues, Maria Gabriela Fontanetti; Oliveira, Natália Batista; Boliani, Aparecida Conceição

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dc.creator	Ferreira, Antonio Flávio Arruda
dc.creator	Monteiro, Laís Naiara Honorato
dc.creator	Rodrigues, Maria Gabriela Fontanetti
dc.creator	Oliveira, Natália Batista
dc.creator	Boliani, Aparecida Conceição
dc.date	2018-08-01
dc.date.accessioned	2023-03-30T19:35:06Z
dc.date.available	2023-03-30T19:35:06Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/2602
dc.identifier	10.14295/cs.v9i2.2602
dc.identifier.uri	https://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/246779
dc.description	The tamarind tree (Tamarindus indica L.) is a common tree in tropical countries with a great exploitation potential due to its high nutritional value and important pharmaceutical characteristics, justifying its potential as a promising crop. The scarcity of scientific studies of the species, especially on propagation, hinders its availability and, consequently, the supply of the product in the market. The aim of this study was to verify the obtainment of nodal segments via sexual propagation and the in vitro establishment of sweet tamarind in MS culture medium (25, 50, 75 and 100% of salts) and with or without activated charcoal (2 g.L-1). The experiment was carried out in a completely randomized design in a 2 x 4 factorial scheme (presence and absence of activated carbon x salt concentrations), with 25 replicates, each replicate consisting of a test tube with an inoculated explant. According to the results, it is possible to conclude that from seedlings with 45 days after sowing, nodal segments of sweet tamarind are obtained for in vitro establishment. As a precursor of protocol for in vitro formation of healthy seedlings is indicated the use of MS culture medium with 75 % of the salts and added with 2 gL-1 of activated charcoal to reduce the contamination index.	en-US
dc.description	The tamarind tree (Tamarindus indica L.) is a common tree in tropical countries with a great exploitation potential due to its high nutritional value and important pharmaceutical characteristics, justifying its potential as a promising crop. The scarcity of scientific studies of the species, especially on propagation, hinders its availability and, consequently, the supply of the product in the market. The aim of this study was to verify the obtainment of nodal segments via sexual propagation and the in vitro establishment of sweet tamarind in MS culture medium (25, 50, 75 and 100% of salts) and with or without activated charcoal (2 g.L-1). The experiment was carried out in a completely randomized design in a 2 x 4 factorial scheme (presence and absence of activated carbon x salt concentrations), with 25 replicates, each replicate consisting of a test tube with an inoculated explant. According to the results, it is possible to conclude that from seedlings with 45 days after sowing, nodal segments of sweet tamarind are obtained for in vitro establishment. As a precursor of protocol for in vitro formation of healthy seedlings is indicated the use of MS culture medium with 75 % of the salts and added with 2 gL-1 of activated charcoal to reduce the contamination index.	pt-BR
dc.format	application/pdf
dc.language	eng
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2602/559
dc.rights	Copyright (c) 2018 Antonio Flávio Arruda Ferreira, Laís Naiara Honorato Monteiro, Maria Gabriela Fontanetti Rodrigues, Natália Batista Oliveira, Aparecida Conceição Boliani	en-US
dc.source	Comunicata Scientiae; Vol. 9 No. 2 (2018); 298-302	en-US
dc.source	Comunicata Scientiae; v. 9 n. 2 (2018); 298-302	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.subject	Tamarind	en-US
dc.subject	tissue culture	en-US
dc.subject	exotic fruit tree	en-US
dc.subject	salt concentration	en-US
dc.subject	active charcoal	en-US
dc.subject	Tamarind	pt-BR
dc.subject	tissue culture	pt-BR
dc.subject	exotic fruit tree	pt-BR
dc.subject	salt concentration	pt-BR
dc.subject	active charcoal	pt-BR
dc.title	In vitro cultivation of Tamarindus indica L.: explants obtention and contamination in culture medium	en-US
dc.title	In vitro cultivation of Tamarindus indica L.: explants obtention and contamination in culture medium	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.type	Nota Científica	en-US