Morphological and molecular characterization of powdery mildew  on watermelon plants in São Paulo state

Leão, Evelynne Urzedo; Rocha, Kelly Cristina Gonçales; Silva, Norberto; Pavan, Marcelo Agenor; Adorian, Gentil Cavalheiro; Krause-Sakate, Renate

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dc.creator	Leão, Evelynne Urzedo
dc.creator	Rocha, Kelly Cristina Gonçales
dc.creator	Silva, Norberto
dc.creator	Pavan, Marcelo Agenor
dc.creator	Adorian, Gentil Cavalheiro
dc.creator	Krause-Sakate, Renate
dc.date	2019-12-31
dc.date.accessioned	2023-03-30T19:35:03Z
dc.date.available	2023-03-30T19:35:03Z
dc.identifier	https://comunicatascientiae.com.br/comunicata/article/view/2315
dc.identifier	10.14295/cs.v10i4.2315
dc.identifier.uri	https://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/246742
dc.description	Powdery mildew is one of the most commom foliar diseases of cultivated and wild cucurbits worldwide. Six different fungal species have been reported to be associated with powdery mildew of cucurbits, such as Podosphaera xanthii and Golovinomyces cichoracearum. The aim of the study was to identify the causal agent of the disease in watermelon plants (Citrullus lanatus) cultivated in greenhouses of the State of São Paulo. DNA total was extracted using Chelex method and the ITS region was amplified via standard PCR reaction using the specific primers PMITS1 and PMITS2. A fragment of 700pb was amplified and the sequence showed 99% of identity with P. xanthii (accession number JQ728480). Morphological observation showed that the conidia were ellipsoidal-ovoid, had fibrosin bodies and a bifurcated germinative tube in the lateral position. These characteristics and the molecular analyses confirmed P. xanthi as the etiological agent of powdery mildew in watermelon plants in São Paulo State. Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state	en-US
dc.description	Powdery mildew is one of the most commom foliar diseases of cultivated and wild cucurbits worldwide. Six different fungal species have been reported to be associated with powdery mildew of cucurbits, such as Podosphaera xanthii and Golovinomyces cichoracearum. The aim of the study was to identify the causal agent of the disease in watermelon plants (Citrullus lanatus) cultivated in greenhouses of the State of São Paulo. DNA total was extracted using Chelex method and the ITS region was amplified via standard PCR reaction using the specific primers PMITS1 and PMITS2. A fragment of 700pb was amplified and the sequence showed 99% of identity with P. xanthii (accession number JQ728480). Morphological observation showed that the conidia were ellipsoidal-ovoid, had fibrosin bodies and a bifurcated germinative tube in the lateral position. These characteristics and the molecular analyses confirmed P. xanthi as the etiological agent of powdery mildew in watermelon plants in São Paulo State.	pt-BR
dc.format	application/pdf
dc.language	eng
dc.publisher	Federal University of Piauí	en-US
dc.relation	https://comunicatascientiae.com.br/comunicata/article/view/2315/874
dc.rights	Copyright (c) 2019 Evelynne Urzedo Leão, Kelly Cristina Gonçales Rocha, Norberto Silva, Marcelo Agenor Pavan, Gentil Cavalheiro Adorian, Renate Krause-Sakate	en-US
dc.source	Comunicata Scientiae; Vol. 10 No. 4 (2019): Comunicata Scientiae; 505-508	en-US
dc.source	Comunicata Scientiae; v. 10 n. 4 (2019): Comunicata Scientiae; 505-508	pt-BR
dc.source	2177-5133
dc.source	2176-9079
dc.title	Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state	en-US
dc.title	Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state	pt-BR
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion