1. Repositorio institucional de CLACSO
2. Colección de los Centros Asociados
3. Brasil
4. Núcleo de Pesquisa sobre Crianças, Adolescestes e Jovens - Universidade Federal do Piauí - NUPEC/UFPI - Cosecha

Título :	Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins Correlações entre atividade inseticida e resistência a proteólise de duas lectinas vegetais glicose/manose
Palabras clave :	Carbohydrate-binding proteins;Callosobruchus maculatus;Dysdercus peruvianus;lectins.;Proteínas ligantes a carboidratos;Callosobruchus maculatus;Dysdercus peruvianus;Pragas agrícolas.
Editorial :	Federal University of Piauí
Descripción :	Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed. Lectinas purificadas de sementes de Canavalia brasiliensis (ConBr) e Cratylia floribunda (CFL) foram testadas quanto a atividade inseticida sobre Callosobruchus maculatus (Coleoptera: Bruchidae) e Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr e CFL causaram efeitos negativos sobre o desenvolvimento das larvas de C. maculatus nas concentrações de 1% e 2% (p/p). O tempo de desenvolvimento dos insetos crescidos nas sementes artificiais contendo ConBr ou CFL não foi significativamente alterado, entretanto a porcentagem de emergência foi diminuída. Ambas lectinas foram resistentes a ação hidrolítica de proteases intestinais de larvas de C. maculatus. Dietas artificiais contendo as lectinas não causaram efeitos deletérios sobre a massa e a sobrevivência de ninfas de D. peruvianus. Proteases intestinais extraídas de D. peruvianus foram capazes de hidrolisar ConBr e CFL após 30 minutos de incubação. A habilidade dos insetos para digerir as lectinas parece estar diretamente relacionada ao efeito inseticida observado. Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/Mannose Plant Lectins AbstractPurified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed.
URI :	http://biblioteca-repositorio.clacso.edu.ar/handle/CLACSO/77477
Otros identificadores :	https://comunicatascientiae.com.br/comunicata/article/view/15 10.14295/cs.v2i1.15
Aparece en las colecciones:	Núcleo de Pesquisa sobre Crianças, Adolescestes e Jovens - Universidade Federal do Piauí - NUPEC/UFPI - Cosecha

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.